@brian: Still I have Black background for inserted .png files in Layout when printing. The .png was created using Rhino Cycles render and saved as png with alpha channel. Also I checked in the material properties and the alpha chennel it is checked.
This is the Layout viewport:
And this is the printing result:
Do you see the problem with any other PNG that has alpha channel, i.e. created with GIMP or Photoshop?
Unfortunatelly seems that yes.
Just made a quick picture in Gimp and saved as png with alpha channel.
OK, I just wanted to rule out any relationship with Raytraced.
Logged as RH-43516.